In this study, the integrated in situ extraction-gas stripping process was coupled with continuous ABE fermentation using immobilized Clostridium acetobutylicum. At the same time, oleyl alcohol was co-currently flowed into the packed bed reactor with the fresh medium and then recycled back to the packed bed reactor after removing butanol in the stripper. A high glucose consumption of 52 g/L and a high butanol productivity of 11 gL-1h-1 were achieved, resulting in a high butanol yield of 0.21 g-butanol/g-glucose. This can be attributed to both the high bacterial activity for solvent production as well as a 3-fold increase in the bacterial density inside the packed bed reactor. Also reported is that 64% of the butanol produced can be recovered by the integrated in situ extraction-gas stripping process. A high butanol productivity and a high glucose consumption were simultaneously achieved.

丙酮-丁醇-乙醇發酵, 固定化細胞, 整合式萃取氣提程序, 垂直式填充床

Acetone-butanol-ethanol (ABE) Fermentation; Immobilized Clostridium Acetobutylicum; Integrated Extraction-gas Stripping; Packed Bed in A Vertical Mode